Lab report 1 gram staining

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Lab report 1 gram staining

Gram devised his technique not for the purpose of distinguishing one type of bacterium from another but to make bacteria more visible in stained sections of lung tissue.

Lab report 1 gram staining

It is used mainly to make a preliminary morphologic identification or to establish that there are significant numbers of bacteria in a clinical specimen. It cannot identify bacteria to the species level, and for most medical conditions, it should not be used as the sole method of bacterial identification.

In clinical microbiology laboratories, it is used in combination with other traditional and molecular techniques to identify bacteria. Some organisms are gram-variable meaning they may stain either negative or positive ; some are not stained with either dye used in the Gram technique and are not seen.

Lab report 1 gram staining

In a modern environmental or molecular microbiology lab, most identification is done using genetic sequences and other molecular techniques, which are far more specific and informative than differential staining. Gram staining has been suggested to be as effective a diagnostic tool as PCR in one primary research report regarding gonococcal urethritis.


Gram-negative bacterial infection and Gram-positive bacterial infection Gram stains are performed on body fluid or biopsy when infection is suspected. Gram stains yield results much more quickly than culturingand is especially important when infection would make an important difference in the patient's treatment and prognosis; examples are cerebrospinal fluid for meningitis and synovial fluid for septic arthritis.

There are four basic steps of the Gram stain: Applying a primary stain crystal violet to a heat-fixed smear of a bacterial culture. Heat fixation kills some bacteria but is mostly used to affix the bacteria to the slide so that they don't rinse out during the staining procedure.

The addition of iodidewhich binds to crystal violet and traps it in the cell Rapid decolorization with ethanol or acetone Counterstaining with safranin.

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These ions penetrate through the cell wall and cell membrane of both gram-positive and gram-negative cells. Iodine is often referred to as a mordantbut is a trapping agent that prevents the removal of the CV—I complex and, therefore, color the cell. The CV—I complexes are washed from the gram-negative cell along with the outer membrane.

The large CV—I complexes become trapped within the gram-positive cell due to the multilayered nature of its peptidoglycan.From the report, in Figure 8, the unknown microbes from petri dish 1 give purple and pink colouration after gram staining, they’re round in shape. A more reliable method to identify cell morphology would be to use special stains to identify specific parts of a microbe like endospores, which is usually present in gram positive bacteria.

Oral microbiology is the study of the microorganisms (microbiota) of the oral cavity and their interactions between oral microorganisms or with the host. The environment present in the human mouth allows the growth of characteristic microorganisms found there.

It provides a source of water and nutrients, as well as a moderate temperature. Resident microbes of the mouth adhere to the teeth and.

Hardy Diagnostics Gram Stain Kits and Reagents are used to stain microorganisms from cultures or specimens by the differential Gram method. Do not ingest, inhale, or allow to come into contact with skin.

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View Lab Report - Micro lab report 1 from MICRO at Clemson University. Lab 1: The Gram Stain China Moore Instructor: Nattasha Vinas Microbiology Sec. 8 February 3, This report%(6). View Lab Report - Lab Report 1 - Gram Staining from MICR at Clemson University.

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